BactoReal® Kit Actinobacillus pleuropneumoniae

Detection of the apxIVA gene of Actinobacillus pleuropneumoniae

Background: Actinobacillus pleuropneumoniae (APP) causes respiratory disease in swine (pig haemorrhagic pleuropneumonia, actinobacillosis). It is a Gram-negative coccobacillus and a pathogen of the respiratory tract. Clinical signs of the acute disease are dyspnea, coughing, anorexia, depression, fever and sometimes vomiting. The disease can progress very rapidly and death can occur within a few hours. Chronic infections are characterized by cough and pleuritis. Many herds are infected with APP without presenting any clinical evidence of the disease. Carrier pigs harbour APP in their nasal cavities and/or tonsils.

Description: BactoReal® Kit Actinobacillus pleuropneumoniae uses real-time PCR for the detection of the apxIVA gene of Actinobacillus pleuropneumoniae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: apxIVA gene of Actinobacillus pleuropneumoniae
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX