Detection of the ITS2 region of the MTBC (Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium caprae, Mycobacterium canetti, Mycobacterium africanum and Mycobacterium microti)
Mycobacterium bovis detection test kit by using qPCR. Mycobacterium bovis is the agent responsible for bovine tuberculosis, however, it can also cause the disease in humans if there is the consumption of infected milk. The pathology of M. bovis is similar to M. tuberculosis in humans, causing chronic debilitation, coughing, and further spread to other organs.
Evaluate the expression of four virulence factors (fedA, faeG, eae and toxA from Escherichia coli) by using qPCR. toxA, coding for the heat-labile enterotoxin (LT); eae, coding for the intimine; fedA, coding for the F18 fimbriae and faeG, coding for the F4 fimbriae. Post-weaning diarrhea is an important enteric disease that usually occurs shortly after weaning and is characterized by watery diarrhea, dehydration, loss of body weight, and the death of infected pigs. This diarrhea is responsible for important economic losses due to mortality, morbidity, decreased growth rate and cost of medication. Escherichia coli is one of the most important causes of the increasing worldwide outbreaks of post-weaning diarrhea, causing sudden death or severe diarrhoea. The PCR identification of virulence factors of E. coli from fecal samples is normally used for the diagnosis of pathogenic isolates.