BactoReal® Kit Haemophilus parasuis/Glaesserella parasuis

Detection of the 16S rRNA gene of Haemophilus parasuis/Glaesserella parasuis

Background: Haemophilus parasuis/Glaesserella parasuis is a Gramnegative bacterium (member of the Pasteurellaceae) of the upper respiratory tract of swine. It is the aetiological agent of Glässers disease, a severe systemic disease characterized by fibrinous polyserositis, pericarditis, arthritis and meningitis. In addition to the acute form of the disease, Haemophilus parasuis is an important component of the Porcine Respiratory Disease Complex (PRDC). Fifteen serovars of H. parasuis have been reported, but a high percentage of the evaluated isolates are nontypeable. Isolates from the upper respiratory tract are mainly apathogenic strains of clinically healthy swine.

Description: BactoReal® Kit Haemophilus parasuis/Glaesserella parasuis uses real-time PCR for the detection of the 16S rRNA gene of H. parasuis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination, the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: 16S rRNA gene of Haemophilus parasuis/Glaesserella parasuis
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX