BactoReal® Kit Listeria monocytogenes

Detection of the hlyA gene of Listeria monocytogenes

Background: Listeria monocytogenes causes listeriosis an infectious but not contagious disease affecting primarily domestic animals, especially ruminants (cattle, sheep, goats) and is less common in human beings. Rarely the disease can be found in birds, horses and pigs as well as in rodents and fish. It can also occur in feral animals (e.g. game animals) as well as in poultry and other birds. Listeria are found ubiquitously in soil, water and in poorly made silage.

Description: BactoReal® Kit Listeria monocytogenes uses real-time PCR for the detection of the hlyA gene of L. monocytogenes. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: hlyA gene of Listeria monocytogenes
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX