BactoReal® Kit Mycoplasma bovis

Detection of the rpoB gene of Mycoplasma bovis

Background: Mycoplasma bovis is a pathogen causing respiratory disease, otitis media, arthritis and mastitis in cattle worldwide.

Description: BactoReal® Kit Mycoplasma bovis uses real-time PCR for the detection of the rpoB gene of M. bovis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: rpoB gene of Mycoplasma bovis
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX