BactoReal® Kit Streptococcus agalactiae

Detection of the cfb gene of Streptococcus agalactiae.

Background: Streptococcus agalactiae (also known as Group B streptococcus or GBS) is a beta-hemolytic Gram-positive streptococcus. It is pathogenic primarily for animals but can also infect humans. Since S. agalactiae strains isolated from animals can be differed from those isolated from humans, transmission from animals to humans is rare. Streptococcus agalactiae is a member of the gastrointestinal normal flora in some humans and can spread to secondary sites including the vagina. For this reason, it can cause sepsis, pneumonia and meningitis in newborns. In persons with a weak immune system, it can cause infections of the urinary tract and of wounds, meningitis and sepsis. Streptococcus agalactiae secretes a protein (CAMP factor) which causes haemolysis in presence of the beta-toxin of Staphylococcus aureus in blood agar.

Description: BactoReal® Kit Streptococcus agalactiae uses real-time PCR for the detection of the cfb gene of Streptococcus agalactiae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination, the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: cfb gene of Streptococcus agalactiae
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or assay-only