Crustaceans (Tropomyosin) ELISA

Enzyme immunoassay for the quantitative determination of Crustacean Tropomyosin in food.

Background: Not only by reason of their cross-reactivity to house dust mites, crustaceans represent an important group of food allergens. In this regard tropomyosin, which can be found in all common crustacean species, is the most important protein. In cooked crustacean extracts this protein represents approximately 20% of total protein. For crustacean allergic persons hidden crustacean proteins in food are a critical problem. Already very low amounts of the allergen can cause allergic reactions, which may lead to anaphylactic shock in severe cases. Because of this, crustacean allergic persons must strictly avoid the consumption of crustacean containing food. Cross-contamination, mostly in consequence of the production process, is often noticed. This explains why in many cases the existence of crustacean residues in food cannot be excluded. For this reason sensitive detection systems for crustacean residues in foodstuffs are required.
The Crustaceans (Tropomyosin) ELISA represents a highly sensitive detection system for tropomyosin (from penaeus indicus) and is particularly capable of the quantification of crustacean residues in fish products, soups, dressings, bakery products and meat products.

Description: The Crustaceans (Tropomyosin) quantitative test is based on the principle of the enzyme linked immunosorbent assay. An antibody directed against tropomyosin is bound on the surface of a microtiter plate. Tropomyosin containing samples or standards are given into the wells of the microtiter plate. After 20 minutes incubation at room temperature, the wells are washed with diluted washing solution to remove unbound material. A peroxidase conjugated second antibody directed against tropomyosin is given into the wells and after 20 minutes of incubation the plate is washed again. A substrate solution is added and incubated for 20 minutes, resulting in the development of a blue colour. The colour development is inhibited by the addition of a stop solution, and the colour turns yellow. The yellow colour is measured photometrically at 450 nm. The concentration of tropomyosin is directly proportional to the colour intensity of the test sample.

Product Features:
- The kit contains reagents for 96 determinations;
- Microtiter plate consisting of 12 strips with 8 breakable wells;
- Tropomyosin Standards: 0, 20, 60, 200, 400 ppb;
- ELISA reader at 450 nm;
- Standard range: 20 - 400 ppb;
- Sensitivity analytical: 0.09 ppb.