Fumonisin ELISA

Enzyme immunoassay for the rapid quantitative determination of Fumonisin in cereals and beer /gyle.

Background: Fumonisin in addition to zearalenone, deoxynivalenol and other trichothecenes belongs to the fusarium toxins. These toxins are already produced on the field in consequence of a contact of the cereals by fusarium species. These toxins show an extreme stability against high temperatures (up to 100°C), and they can remain active in contaminated food for years. Fumonisin can be found in maize, oats and other types of grain. Worldwide a contamination in maize of 60% has been detected. When ingested by animals, fumonisin leads to neurotoxicity, hepatotoxicity and lung edema, mainly in horses and pigs. Therapeutic measures are the change of the grain given to the animals, or the administration of diuretic drugs. In human patients hints for the appearance of esophagus cancer could be associated with the exposition to fumonisin B1. Values assessed for the acute toxicity are 8 mg per kg weight and for the chronic situation 25 mg/kg in feed stuff. Since June 2010 the US Food and Drug Association recommends maximum amounts of 2 - 100 ppm for raw cereals depending on the intended use. In the European Union the limits are 0.1 – 0.5 ppm for food and 5-60 ppm for feed products. Thus a monitoring of food and feed with respect to the concentration of fumonisin is obligatory.
The Fumonisin ELISA represents a highly sensitive detection system and is particularly capable of the rapid quantification of fumonisin contaminations in cereals and beer.

Description: The Fumonisin ELISA quantitative test is based on the principle of the enzyme-linked immunosorbent assay. An antibody binding protein is coated on the surface of a microtiter plate. Fumonisin containing samples or standards, a fumonisin-peroxidase conjugate and an antibody directed against fumonisin are given into the wells of the microtiter plate. The conjugate competes with the fumonisin of samples/standards for the limited number of antibody sites. Simultaneously the anti-fumonisin antibody is bound to the antibody-binding protein coated on the microtiter plate. After 10 minutes incubation at room temperature the wells are washed with diluted washing solution to remove unbound material. A substrate solution is added and incubated for 10 minutes, resulting in the development of a blue colour. The colour development is inhibited by the addition of a stop solution, and the colour turns yellow. The yellow colour is measured photometrically at 450 nm. The concentration of fumonisin is indirectly proportional to the colour intensity of the test sample.

Product Features:
- The kit contains reagents for 96 determinations;
- Microtiter plate consisting of 12 strips with 8 breakable wells;
- Fumonisin (B1) Standards: 0, 0.05, 0.15, 0.5, 1.5, 5 ppm;
- ELISA reader at 450 nm;
- Standard range: 0.05 - 5 ppm;
- Sensitivity analytical: 0.015 ppm.