MycoReal Kit Aspergillus

Detection of ITS2-region of Aspergillus.

Background: Aspergillus is a fungal genus consisting of several hundred species. Aspergillus species are ubiquitously found and some species can cause opportunistic infections such as allergic bronchopulmonary aspergillosis, pulmonary aspergilloma and invasive aspergillosis. Invasive aspergillosis is increasingly recognized as a primary cause of morbidity and mortality especially in immunocompromised patients. Aspergillus fumigatus is the predominant disease-causing species. However, other species than A. fumigatus, in particular A. terreus and A. flavus but also A. niger, A. nidulans and A. ustus have gained greater clinical significance. Not only the detection, but also the identification of the fungal species is important. The species might provide information on a potential contamination, colonisation or infection and moreover antifungal resistance.

Description: MycoReal Kit Aspergillus is a real-time PCR test for the detection of Aspergillus fumigatus, Aspergillus flavus, Aspergillus terreus, Aspergillus niger and Aspergillus nidulans from samples purified from bronchoalveolar lavages (BAL), blood, aspirates, cerebrospinal fluid and tissue. Furthermore, it detects some other closely related Aspergillus/Emericella species belonging to Aspergillus section Fumigati, Flavi, Terrei, Nigri, Nidulantes, Usti and Versicolori. The kit detects the internal transcribed spacer 2 (ITS2-region, multicopy gene) of Aspergillus. Due to the use of specific probes (FAM and VIC labelled) the test is highly specific for Aspergillus. It allows the detection and to some extent also the distinction of medically important Aspergillus species. Analysis is done with Assay Mix 1 and Assay Mix 2 in two parallel PCR-reactions. The internal DNA positive control (DNA IPC) is detected in Cy5 channel and is used as DNA extraction as well as PCR inhibition control. The DNA IPC target is extracted with the sample.

Product Features:
- Amplification and detection: ITS2-region of Aspergillus
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously