MycoReal® Kit Fungi

Universal (broad-range) detection and identification of DNA of fungi (ITS2-region).

Background: Fungal diseases are mostly opportunistic infections with a great variety of common and uncommon pathogens. Up to 150 fungal species have been shown as potential human pathogens involving all body sites. Invasive mycoses are increasingly recognized as a primary cause of morbidity and mortality especially in immunocompromised patients. These infections are mainly caused by yeasts (such as Candida spp.,Cryptococcus neoformans, Saccharomyces cerevisiae, Trichosporon spp.) and moulds (such as Aspergillusspp., Scedosporium spp., Fusarium spp., Natrassia mangiferae, Curvularia spp., Schizophyllum commune, Paecilomyces variotii, Bipolaris spp., Cladophialophora bantiana, and zygomycetes such as Rhizopus spp.,Absidia spp., Rhizomucor spp., Mucor spp., Cunninghamella bertholletiae, Saksenaea vasiformis, Apophysomyces elegans, Basidiobolus ranarum, Conidiobolus spp.). The mortality rate of invasive fungal infections is 40-100%. A rapid diagnosis improves the outcome. Non-invasive fungal infections (such as infections of the urogenitary tract, of the eye, of the skin, etc.) are mainly caused by Candida, Aspergillus, Acremonium, Fusarium and dermatophytes (such as Trichophyton spp., Microsporum spp.).

Description: MycoReal® Kit Fungi is a real-time PCR test and detects the ITS2-region of fungi. The ITS2-region can be universally found in fungi and is therefore a proper target for the broad-range (panfungal) detection of fungal DNA.

Product Features:
- Amplification and detection: ITS2 region of fungi
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available format: Kit