Real Time PCR Detection Kit Actinobacillus pleuropneumoniae Apx Toxin-type Panel (ApxI ApxII and ApxIII)

Background:The panel comprises a series of species-specific targeted reagents designed for the detection of the three Apx toxins of the Actinobacillus pleuropneumoniae (ApxI, ApxII and ApxIII) by using qPCR.
Actinobacillus pleuropneumoniae (previously Haemophilus pleuropneumoniae), is a non-motile, Gram-negative, encapsulated coccobacillus bacterium found in the Pasteurellaceae family. It is a respiratory pathogen found in pigs and was found to be the causative agent for up to 20% of all bacterial pneumonia cases in swine. The main disease associated with this bacterium is porcine pleuropneumonia, a highly contagious respiratory disease, affecting primarily young pigs (usually less than 6 months). The symptoms include respiratory distress, bloodstained discharge (usually frothy) from the mouth, fever, anorexia, mild diarrhea, cyanosis, lethargy, and spontaneous abortion in sows. Mortality rates can reach 20-80 % in fattening pigs, with similarly high morbidity. Pigs that do survive the disease remain as carriers and spread the bacterium to other swine. A. pleuropneumoniae has a profound economic impact on pork production and pig farmers. Two biotypes and at least 15 serotypes are present. Several virulence factors account for the remarkable pathogenicity of A. pleuropneumoniae. One of the more important is the production and release of the Apx toxins, which are subdivided into four types: ApxI through ApxIV (ApxIV is expressed in all serotypes). Each serotype is described to express different amounts of the four Apx toxins.

Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
-Standard Template, dehydrated target copies for positive control