Real Time PCR Detection Kit Escherichia coli virulence factor genes Panel (Fimbria F5 (K99) Fimbria F17 and Fimbria F41)

Detection test kit of 10 different virulence genes from Escherichia coli by using qPCR. Some E. coli are pathogenic, meaning they can cause illness, either diarrhea or illness outside of the intestinal tract. The types of E. coli that can cause diarrhea can be transmitted through contaminated water or food, or through contact with animals or persons. Virulence genes detected include the Aerobactin synthase (iucC), Ligand-gated channel protein (fyuA), Ferric enterobactin receptor (iroN) and Aerobaction siderophore ferric receptor (iutA), all of them involved in iron transport under iron-stressed conditions; Temperature-sensitive hemagglutinin tsh autotransporter (tsh) can act both as an adhesin and as a serine protease; Colicin-V immunity protein (cvi) protects a cell against colicin V; Protein iss (iss) increases serum survival and confers group II surface exclusion; Chaperone protein (fimC) required for the biogenesis of type 1 fimbriae; ompT protein (ompT) aspartyl protease found on the outer membrane and Hemolysin F (hlyF) fatty-acyl-CoA reductase activity.

Evaluate the expression of four virulence factors (fedA, faeG, eae and toxA from Escherichia coli) by using qPCR. toxA, coding for the heat-labile enterotoxin (LT); eae, coding for the intimine; fedA, coding for the F18 fimbriae and faeG, coding for the F4 fimbriae. Post-weaning diarrhea is an important enteric disease that usually occurs shortly after weaning and is characterized by watery diarrhea, dehydration, loss of body weight, and the death of infected pigs. This diarrhea is responsible for important economic losses due to mortality, morbidity, decreased growth rate and cost of medication. Escherichia coli is one of the most important causes of the increasing worldwide outbreaks of post-weaning diarrhea, causing sudden death or severe diarrhoea. The PCR identification of virulence factors of E. coli from fecal samples is normally used for the diagnosis of pathogenic isolates.

Detection test kit of Campylobacter coli by using qPCR. Campylobacter coli usually lives in the intestinal tract of animals. C. coli is very similar to its bacteria relative, Campylobacter jejuni; both cause inflammation of the intestine and cause diarrhea in infected animals and humans. C. coli is of the worldwide prevalence and is common in both developed and developing countries with most outbreaks related to food or water-borne causes.

Detection test kit of Escherichia coli by using qPCR. Some E. coli are pathogenic, meaning they can cause illness, either diarrhea or illness outside of the intestinal tract. The types of E. coli that can cause diarrhea can be transmitted through contaminated water or food, or through contact with animals or persons.

Caprine mycoplasmas Mycoplasma spp., Mycoplasma ovipneumoniae and Mycoplasma capricolum detection test kit by using qPCR. They parasitize, on the surface or intracellularly, a wide range of organisms including humans, plants, animals, and insects. M. ovipneumoniae is a respiratory pathogen of domestic sheep, domestic goats, bighorn sheep, mountain goats, and another caprine that can both cause primary atypical pneumonia and predispose infected animals to secondary pneumonia with other agents. M. capricolum subsp. capripneumoniae is the etiologic agent of contagious caprine pleuropneumonia, a severe and typically lethal respiratory disease. M. capricolum subsp. capricolum infection is usually non-fatal and instead results in chronic inflammation in a variety of organs, including joints, udder, eyes, and lungs. M. capricolum subsp. capricolum infection occurs worldwide and appears widespread but has rarely been found in species of small ruminants other than domestic goats and, occasionally, sheep.