Real Time PCR Detection Kit Escherichia coli Virulence Genes Panel

Background:Detection of 10 different virulence genes from Escherichia coli by using qPCR.
Escherichia coli (E. coli) bacteria normally live in the intestines of people and animals. Most E. coli are harmless and actually are an important part of a healthy human intestinal tract. However, some E. coli are pathogenic, meaning they can cause illness, either diarrhea or illness outside of the intestinal tract. The types of E. coli that can cause diarrhea can be transmitted through contaminated water or food, or through contact with animals or persons. Virulence genes detected include the Aerobactin synthase (iucC), Ligand-gated channel protein (fyuA), Ferric enterobactin receptor (iroN) and Aerobaction siderophore ferric receptor (iutA), all of them involved in iron transport under iron-stressed conditions; Temperature-sensitive hemagglutinin tsh autotransporter (tsh) can act both as an adhesin and as a serine protease; Colicin-V immunity protein (cvi) protects a cell against colicin V; Protein iss (iss) increases serum survival and confers group II surface exclusion; Chaperone protein (fimC) required for the biogenesis of type 1 fimbriae; ompT protein (ompT) aspartyl protease found on the outer membrane and Hemolysin F (hlyF) fatty-acyl-CoA reductase activity. Additionally, the kit includes reagents for E. coli detection as a control.

Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
-Standard Template, dehydrated target copies for positive control