Cell-free human DNA detection by using qPCR. The target is a multiple-copy gene, 200 copies per genome, with a slow evolutionary rate. Due to the multiple-copy and the high degree of conservation, can be used for quantification of low amounts of circulating cell-free DNA (cfDNA) present in human blood. Cell-free DNA is becoming an important clinical analyte for prenatal testing, cancer diagnosis, and cancer monitoring.
1.000000002563615
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