Real Time PCR Detection Kit Shiga toxin 2 Escherichia coli

Background:Shiga toxin 2 Escherichia coli detection by using qPCR.
Shiga toxin is a family of related toxins with two major groups, Stx1 and Stx2. The most common sources of Shiga toxin are the bacteria Shigella dysenteriae and the Shiga toxigenic group of Escherichia coli (STEC), which includes serotypes O157:H7, O104:H4, and other enterohemorrhagic E. coli (EHEC). Shiga toxins have many names, which can be used interchangeably (shiga toxins, shiga-like toxins, cytotoxins, verocytotoxins, verotoxins). STEC lives in the guts of ruminant animals, including cattle, goats, sheep, deer, and elk. The major source of human illnesses is cattle, where STEC generally does not make animals become sick. The symptoms of STEC infections often include severe stomach cramps, diarrhea (often bloody), and vomiting. Most patients recover within 10 days, but in young children and the elderly, the infection may lead to a life-threatening disease, such as hemolytic uraemic syndrome. Haemolytic uraemic syndrome is characterized by acute renal failure, hemolytic anemia, and thrombocytopenia. Stx-2 has been found to be about 400 times more toxic than Stx-1.

Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
-Standard Template, dehydrated target copies for positive control