REAL TIME PCR DETECTION TEST KIT FOOD SAFETY Lupin

Detection of Lupin DNA in food products and environmental samples. The kit is suitable for use in real time instruments with FAM and ROX detection channels.

BACKGROUND: The severity of the reactions triggered by food allergens vary from mild urticarial to potentially lethal anaphylactic shocks, even after the ingestion of minimal doses of the allergenic food. The prevalence of clinically confirmed food allergy indicates that up to 2.5% of the adult population and 6–8% of children less than 3 years of age are affected by food allergies. However, population surveys have estimated the rate of prevalence of perceived food allergy in adults to be between 12 and 20%. This confirms that food allergy represents a major threat to human health. Within the European Union, the presence of the most important food allergens needs to be declared on the label of food products whenever they are used as ingredients as required by Directive 2006/142/EC and is maintained in the recent Directive 2007/68/EC. Lupin is one of the allergens listed in this directive.
Several methods for allergen detection are based on the identification of proteins, especially by means of immunological methods. However, these methods are not reliable for application in highly processed and heated products due to protein deterioration. DNA is more stable than proteins during processing and although it can be fragmented by several processes, it continues to be detectable by PCR. Real time PCR techniques are especially suitable for these products because small fragments of DNA can still be amplified and identified with high sensitivity and specificity.

PRODUCT DESCRIPTION: REAL TIME DETECTION Lupin uses real-time PCR for the detection of Lupin in a simple, reliable, and rapid procedure. The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the target as well as a positive control and an internal control (IC).

PRODUCT FEATURES:
-Amplification and detection of Lupin
-Real-time PCR with rapid hot-start Taq DNA polymerase
-Internal Control to exclude false-negative results
-PCR- platforms: runs on all established standard real-time PCR- platforms
-Harmonized thermal profiles to run with other BIOPREMIER kits simultaneously
-Channel Target: FAM, Channel IC: ROX
-Available formats: Kits with 100 or 10 reactions