Search
EN

Real Time Pcr Detection Test Kit Supreme Escherichia Coli O157:H7 / O157 (Duplex)

Brand: Biopremier
Food PCR test detection of E. coli O157 and allows the simultaneous Food PCR test detection of the serotype O157:H7 in food products, animal feedstuff and environmental samples. Pre-enrichment according to ISO/TS 13136 is recommended or from E. coli isolated colonies. For use in real time instruments with FAM, HEX/VIC and ROX channels.
SKU: BIOPSFS-0059
*

Register or Log In in order to request a quote

Register Login

Products specifications
TechnologyqPCR / Real Time PCR/ PCR
SizesBIOPSFS-0059: 100 reactions - FAM, HEX, ROX
BIOPSFS-sp0059: 10 reactions - FAM, HEX, ROX
Technical SheetDownload PDF

Detection of E. coli O157 and allows the simultaneous detection of the serotype O157:H7 in food products, animal feedstuff and environmental samples. Pre-enrichment according to ISO/TS 13136 is recommended or from E. coli isolated colonies. For use in real time instruments with FAM, HEX/VIC and ROX channels.

BACKGROUND: Escherichia coli (E. coli) is a bacterium that is commonly found in the gut of humans and warm-blooded animals and most of the strains are harmless. Some strains however, such as enterohemorrhagic E. coli (EHEC) O157:H7 can cause serious foodborne outbreaks that causes diarrhoea, fever and vomiting in humans.
E. coli O157:H7 is recognized by its somatic (cell wall) antigen (O157) and its flagella antigen (H7). In addition, E. coli O157:H7 is known to produce Shiga-like toxins, which cause severe symptoms.
The reservoir of this pathogen appears to be mainly cattle, in addition to others such as ruminants, mammals and birds E. coli O157:H7 is transmitted to humans primarily through consumption of contaminated foods, such as raw or undercooked ground meat products and raw milk. Faecal contamination of water and other foods, as well as cross-contamination during food preparation (with beef and other meat products, contaminated surfaces and kitchen utensils), will also lead to infection.
The need for rapid, accurate, and sensitive methods for the detection of these E. coli strains is a major food safety issue. Since conventional microbiological methods for their detection and identification are time-consuming. Today, there are approved methods for PCR based detection of pathogenic genes of Escherichia coli unique DNA sequences, in particular using real time PCR and specific fluorescent probes.

PRODUCT DESCRIPTION: SUPREME REAL TIME DETECTION E. coli O157:H7 / O157 uses real-time PCR based on the use of TaqMan technology for the detection of pathogenic E. coli O157 and the simultaneous detection of the serotype O157:H7 DNA, providing a simple, reliable and rapid result for the detection of E. coli O157:H7 / O157 infection.
The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the target as well as a positive control and an internal control (IC). To minimize PCR cross-contamination,the Kit contains dUTP and uracil-N glycosylase (UDG)

PRODUCT FEATURES:
-Amplification and detection: E. coli O157 and the serotype H7
-Real-time PCR with rapid hot-start Taq DNA polymerase
-Internal Control to exclude false-negative results
-Optimized to handle PCR inhibitors, containing UDG enzyme
-PCR- platforms: runs on all established standard real-time PCR- platforms
-Harmonized thermal profiles to run with other SUPREME BIOPREMIER kits simultaneously
-Channel Targets: FAM and HEX/VIC, Channel IC: ROX
-Available formats: Kits with 100 or 10 reactions

Similar Products

Real Time PCR Detection Kit Escherichia coli O157:H7

Detection test kit of Escherichia coli O157:H7 by using qPCR. Escherichia coli O157:H7 is an enterohemorrhagic strain of the bacterium Escherichia coli and a cause of illness through food. Infection may lead to hemorrhagic diarrhea, and to kidney failure. Transmission is via the fecal-oral route, and most illness has been through undercooked, contaminated ground beef or ground pork being eaten.

Real Time PCR Detection Test Kit Escherichia Coli Supreme (Stx1 Stx2 and Eae Genes)

Food PCR test detection of E. coli pathogenic genes (stx1 , stx2 and eae) in food products, animal feedstuff and environmental samples. Pre-enrichment according to ISO/TS 13136:2012 (recommended). For use in real time instruments with FAM and ROX channels. AOAC certified

Real Time Pcr Detection Test Kit Escherichia Coli Serotype / Serogroup (O157 O26 O111 O103 And O145)

Food PCR test detection of E. coli serotypes O157, O26, O111, O103, and O145 in food products, animal feedstuff and environmental samples. Pre-enrichment according to ISO/TS 13136 is recommended or from E. coli isolated colonies. For use in real time instruments with FAM, and ROX channels.

REAL TIME PCR DETECTION TEST KIT FOOD SAFETY Escherichia coli (EPEC VTEC and EHEC)

Food PCR test detection of E. coli pathogenic genes (stx1 , stx2 and eae) in food products, animal feedstuff and environmental samples. Pre-enrichment according to ISO 16654:2001 (recommended). For use in real time instruments with FAM and ROX channels.

Real Time PCR Detection Kit Shiga toxin 1 Escherichia coli

Shiga toxin 1 Escherichia coli detection test kit by using qPCR. Shiga toxin is a family of related toxins with two major groups, Stx1 and Stx2. Shiga toxigenic group of Escherichia coli (STEC), which includes serotypes O157:H7, O104:H4, and other enterohemorrhagic E. coli (EHEC). Shiga toxins have many names, which can be used interchangeably (shiga toxins, shiga-like toxins, cytotoxins, verocytotoxins, verotoxins). STEC lives in the guts of ruminant animals, including cattle, goats, sheep, deer, and elk. The major source of human illnesses is cattle, where STEC generally does not make animals become sick.