Incubation with the RNase-free DNase I set ensures complete DNA removal from RNA solutions as this enzyme hydrolyzes phosphodiester bonds of dsDNA, ssDNA, chromatin, and DNA-RNA hybrid molecules, without compromising RNA yield and integrity.
PCR purification/Gel extraction: Enzymatic PCR Clean-Up kit, comprising Exonuclease I (Exo I) and recombinant Shrimp Alkaline Phosphatase (rSAP) in an optimal molar ratio. Unused primers are hydrolyzed by Exo I, whilst rSAP dephosphorylates excess dNTPs. 100% recovery, even for very short PCR products
Ribonuclease A is a pancreatic endoribunuclease that specifically cleaves single-stranded RNA at the 3’ end of pyrimidine residues. Lyophilized powder purified from bovine pancreas.