ViroReal® Kit bovine viral diarrhea (BVD) Virus

Detection of RNA of the 5´UTR of bovine viral diarrhea virus (BVDV-1, BVDV-2 and BVDV-3) and of some strains of the classical swine fever virus (CSFV) and border disease virus of sheep (BDV) (FLI approved)

Background: The genus Pestivirus is composed of four important pathogens: bovine viral diarrhea virus 1 and 2 (BVDV-1 and BVDV-2), classical swine fever virus (CSFV), and border disease virus of sheep (BDV). Recently a new putative pestivirus species called “HoBi-like,” “BVDV-3,” or “atypical pestivirus,” was identified. The BVD virus is a single strain, positive-sense RNA virus found throughout the world and causes bovine viral diarrhoea (BVD). BVD is a disease of cattle, which causes various clinical syndromes including diarrhea, mucosal disease, reproductive dysfunctions and/or hemorrhagic syndrome. Prenatal infection by BVD results in cattles permanently infected (BVD-PI). Such animals carry the virus throughout their lifetime and constantly secrete large numbers of viral particles.

Description: ViroReal® Kit BVD Virus uses one-step reverse transcription RT real-time PCR for the detection of 5´UTR of BVDV-1, BVDV-2 and BVDV-3. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

Product Features:
- Amplification and detection: 5´UTR of BVD Virus
- Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
- Contains RNase Inhibitor to block RNA degradation
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: Cy5/ VIC/HEX
- Kit approved by Friedrich-Loeffler-Institute (Nr. FLI-B661)