ViroReal® Kit Schmallenberg Virus

Detection of RNA of the nucleocapsid protein gene of schmallenberg virus (SBV)

Background: Schmallenberg virus (SBV) is a novel Orthobunyavirus that has been associated with disease in ruminants (cattle, sheep and goats) in Europe. It causes transient clinical symptoms in adult cattle and congenital malformation in newborn ruminants. Schmallenberg virus is part of the Simbu serogroup of the Bunyaviridae family, genus Orthobunyavirus. The virus is transmitted via insect vectors, mainly midges.

Description: ViroReal® Kit Schmallenberg Virus uses one-step reverse transcription RT real-time PCR for the detection of the nucleocapsid protein gene of schmallenberg virus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

Product Features:
- Amplification and detection: nucleocapsid protein gene of Schmallenberg Virus
- Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
- Contains RNase Inhibitor to block RNA degradation
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX *400 reactions also available (ref: DVEV03213x4)