BactoReal® Kit Borrelia burgdorferi sensu lato

Detection of the ospA gene of Borrelia species belonging to the Borrelia burgdorferi sensu lato complex (Borrelia spielmanii is not well detected)

Background: Borrelia are Gramnegative bacteria belonging to the Spirochaetaceae. Borrelia burgdorferi sensu stricto, B. garinii, B. afzelii, B. valaisiana, B. lusitaniae and B. spielmanii belong to the B. burgdorferi sensu lato-complex and cause Lyme disease (Lyme borreliosis). These species are associated with different genospecies specific symptoms. They are transmissed by ticks, mainly by Ixodes ricinus. Lyme disease is a multisystemic illness, which can affect various organs, especially the nervous system, skin, heart and joints.

Description: BactoReal® Kit Borrelia burgdorferi sensu lato uses real-time PCR for the detection of the ospA gene of Borrelia species belonging to the Borrelia burgdorferi sensu lato complex. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG) to minimize PCR cross-contamination.

Product Features:
- Amplification and detection: ospA gene of Borrelia burgdorferi sensu lato
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX